BAIT
UBE2G2
UBC7
ubiquitin-conjugating enzyme E2G 2
GO Process (5)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TRIM3
BERP, HAC1, RNF22, RNF97
tripartite motif containing 3
GO Process (1)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system.
Covalent attachment of ubiquitin to substrates is crucial to protein degradation, transcription regulation and cell signalling. Highly specific interactions between ubiquitin-conjugating enzymes (E2) and ubiquitin protein E3 ligases fulfil essential roles in this process. We performed a global yeast-two hybrid screen to study the specificity of interactions between catalytic domains of the 35 human E2s with 250 RING-type E3s. Our ... [more]
Mol. Syst. Biol. Aug. 20, 2009; 5(0);295 [Pubmed: 19690564]
Throughput
- High Throughput
Curated By
- BioGRID