BAIT

UBE2U

RP4-636O23.1
ubiquitin-conjugating enzyme E2U (putative)
UPS Project
Homo sapiens
PREY

TRIM3

BERP, HAC1, RNF22, RNF97
tripartite motif containing 3
UPS Project
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system.

van Wijk SJ, de Vries SJ, Kemmeren P, Huang A, Boelens R, Bonvin AM, Timmers HT

Covalent attachment of ubiquitin to substrates is crucial to protein degradation, transcription regulation and cell signalling. Highly specific interactions between ubiquitin-conjugating enzymes (E2) and ubiquitin protein E3 ligases fulfil essential roles in this process. We performed a global yeast-two hybrid screen to study the specificity of interactions between catalytic domains of the 35 human E2s with 250 RING-type E3s. Our ... [more]

Mol. Syst. Biol. Aug. 20, 2009; 5(0);295 [Pubmed: 19690564]

Throughput

  • High Throughput

Curated By

  • BioGRID