An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers.

Li T, Santockyte R, Shen RF, Tekle E, Wang G, Yang DC, Chock PB

Ubiquitin-like modifiers (UBLs) contain ubiquitin homology domains and can covalently modify target proteins in a manner similar to ubiquitylation. In this study, we revealed a general proteomic approach to elucidate the enzymatic pathways and identify target proteins for three UBLs: SUMO-2, SUMO-3, and NEDD8. Expression plasmids containing the cDNAs of Myc/6xHis doubly-tagged processed or non-conjugatable forms of these UBLs were ... [more]

Arch. Biochem. Biophys. Sep. 01, 2006; 453(1);70-4 [Pubmed: 16620772]

Throughput

High Throughput

Additional Notes

#LPPI
Likely protein-protein interaction

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SAE1 SUMO2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1447315
SUMO2 SAE1	Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.	Brueninghoff K (2020)	High	-	BioGRID	2878376

Curated By

BioGRID

Interaction Summary

SUMO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

SAE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent protein binding [IDA]SUMO activating enzyme activity [IDA]enzyme activator activity [TAS]protein C-terminus binding [IDA]protein binding [IPI]protein heterodimerization activity [IPI, TAS]ubiquitin activating enzyme activity [TAS]