BAIT

SUMO3

SMT3A, SMT3H1, SUMO-3, Smt3B
small ubiquitin-like modifier 3
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

UBA2

ARX, SAE2, HRIHFB2115
ubiquitin-like modifier activating enzyme 2
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers.

Li T, Santockyte R, Shen RF, Tekle E, Wang G, Yang DC, Chock PB

Ubiquitin-like modifiers (UBLs) contain ubiquitin homology domains and can covalently modify target proteins in a manner similar to ubiquitylation. In this study, we revealed a general proteomic approach to elucidate the enzymatic pathways and identify target proteins for three UBLs: SUMO-2, SUMO-3, and NEDD8. Expression plasmids containing the cDNAs of Myc/6xHis doubly-tagged processed or non-conjugatable forms of these UBLs were ... [more]

Arch. Biochem. Biophys. Sep. 01, 2006; 453(1);70-4 [Pubmed: 16620772]

Throughput

  • High Throughput

Additional Notes

  • #LPPI
  • Likely protein-protein interaction

Curated By

  • BioGRID