BAIT

BIM1

YEB1, microtubule-binding protein BIM1, EB1, L000003272, YER016W
Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
Saccharomyces cerevisiae (S288c)
PREY

YME1

OSD1, YTA11, i-AAA protease YME1, L000002522, YPR024W
Catalytic subunit of the i-AAA protease complex; complex is located in the mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as a nonconventional translocation motor to pull PNPase into the intermembrane space; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis.

Vizeacoumar FJ, van Dyk N, S Vizeacoumar F, Cheung V, Li J, Sydorskyy Y, Case N, Li Z, Datti A, Nislow C, Raught B, Zhang Z, Frey B, Bloom K, Boone C, Andrews BJ

We describe the application of a novel screening approach that combines automated yeast genetics, synthetic genetic array (SGA) analysis, and a high-content screening (HCS) system to examine mitotic spindle morphogenesis. We measured numerous spindle and cellular morphological parameters in thousands of single mutants and corresponding sensitized double mutants lacking genes known to be involved in spindle function. We focused on ... [more]

J. Cell Biol. Jan. 11, 2010; 188(1);69-81 [Pubmed: 20065090]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: spindle morphology (APO:0000213)

Additional Notes

  • High Throughput: A synthetic genetic array high-content screening analysis (SGA-HCS) was performed to identify defects in spindle morphogenesis apparent in a double mutant background involving either bni1 or bim1.
  • Low Throughput: Selected double mutants identified using the SGA-HCS analysis were further inspected manually.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BIM1 YME1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1286BioGRID
374607

Curated By

  • BioGRID