Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network.

Markson G, Kiel C, Hyde R, Brown S, Charalabous P, Bremm A, Semple J, Woodsmith J, Duley S, Salehi-Ashtiani K, Vidal M, Komander D, Serrano L, Lehner P, Sanderson CM

In eukaryotic cells the stability and function of many proteins are regulated by the addition of ubiquitin or ubiquitin-like peptides. This process is dependent upon the sequential action of an E1-activating enzyme, an E2-conjugating enzyme, and an E3 ligase. Different combinations of these proteins confer substrate specificity and the form of protein modification. However, combinatorial preferences within ubiquitination networks remain ... [more]

Genome Res. Oct. 01, 2009; 19(10);1905-11 [Pubmed: 19549727]

Quantitative Score

4.0 [Confidence Score]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
XIAP UBE2W	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Dynek JN (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UBE2W

Gene Ontology Biological Process

Gene Ontology Molecular Function

small conjugating protein transferase activity [IBA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IPI]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

XIAP

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]protein binding [IPI]ubiquitin-protein transferase activity [IDA]