BAIT

KAR2

GRP78, Hsp70 family ATPase KAR2, BIP, L000000887, YJL034W
ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

PBP1

MRS16, L000003967, YGR178C
Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
GO Process (4)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

J domain co-chaperone specificity defines the role of BiP during protein translocation.

Vembar SS, Jonikas MC, Hendershot LM, Weissman JS, Brodsky JL

Hsp70 chaperones can potentially interact with one of several J domain-containing Hsp40 co-chaperones to regulate distinct cellular processes. However, features within Hsp70s that determine Hsp40 specificity are undefined. To investigate this question, we introduced mutations into the ER-lumenal Hsp70, BiP/Kar2p, and found that an R217A substitution in the J domain-interacting surface of BiP compromised the physical and functional interaction with ... [more]

J. Biol. Chem. Jul. 16, 2010; 285(29);22484-94 [Pubmed: 20430885]

Quantitative Score

  • -0.63875 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: protein/peptide accumulation (APO:0000149)

Additional Notes

  • Kar2-R217A allele, double mutant shows a synthetic effect in UPR response

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KAR2 PBP1
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High2.5133BioGRID
442016

Curated By

  • BioGRID