BAIT

KAR2

GRP78, Hsp70 family ATPase KAR2, BIP, L000000887, YJL034W
ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

ALG6

dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase, L000003977, YOR002W
Alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
Saccharomyces cerevisiae (S288c)

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

J domain co-chaperone specificity defines the role of BiP during protein translocation.

Vembar SS, Jonikas MC, Hendershot LM, Weissman JS, Brodsky JL

Hsp70 chaperones can potentially interact with one of several J domain-containing Hsp40 co-chaperones to regulate distinct cellular processes. However, features within Hsp70s that determine Hsp40 specificity are undefined. To investigate this question, we introduced mutations into the ER-lumenal Hsp70, BiP/Kar2p, and found that an R217A substitution in the J domain-interacting surface of BiP compromised the physical and functional interaction with ... [more]

J. Biol. Chem. Jul. 16, 2010; 285(29);22484-94 [Pubmed: 20430885]

Quantitative Score

  • 2.06245 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: protein/peptide accumulation (APO:0000149)

Additional Notes

  • Kar2-DAmP allele, double mutant shows a synthetic effect in UPR response
  • Kar2-R217A allele, double mutant shows a synthetic effect in UPR response

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KAR2 ALG6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2532BioGRID
1992076
ALG6 KAR2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3539BioGRID
2069150

Curated By

  • BioGRID