BAIT

KAR2

GRP78, Hsp70 family ATPase KAR2, BIP, L000000887, YJL034W
ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
Saccharomyces cerevisiae (S288c)
PREY

SPF1

COD1, PER9, PIO1, ion-transporting P-type ATPase SPF1, L000003454, YEL031W
P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
Saccharomyces cerevisiae (S288c)

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

J domain co-chaperone specificity defines the role of BiP during protein translocation.

Vembar SS, Jonikas MC, Hendershot LM, Weissman JS, Brodsky JL

Hsp70 chaperones can potentially interact with one of several J domain-containing Hsp40 co-chaperones to regulate distinct cellular processes. However, features within Hsp70s that determine Hsp40 specificity are undefined. To investigate this question, we introduced mutations into the ER-lumenal Hsp70, BiP/Kar2p, and found that an R217A substitution in the J domain-interacting surface of BiP compromised the physical and functional interaction with ... [more]

J. Biol. Chem. Jul. 16, 2010; 285(29);22484-94 [Pubmed: 20430885]

Quantitative Score

  • 3.8116 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • protein/peptide accumulation (APO:0000149)

Additional Notes

  • Kar2-R217A allele, double mutant shows a synthetic effect in UPR response

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPF1 KAR2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.6163BioGRID
2038767
SPF1 KAR2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
211713
KAR2 SPF1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-9.3692BioGRID
209053

Curated By

  • BioGRID