BAIT

ID3

HEIR-1, bHLHb25
inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Homo sapiens
PREY

NKX2-5

CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3
NK2 homeobox 5
GO Process (37)
GO Function (11)
GO Component (3)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

p204 protein overcomes the inhibition of the differentiation of P19 murine embryonal carcinoma cells to beating cardiac myocytes by Id proteins.

Ding B, Liu CJ, Huang Y, Yu J, Kong W, Lengyel P

We reported in the accompanying article (Ding, B., Liu, C., Huang, Y., Hickey, R. P., Yu, J., Kong, W., and Lengyel, P. (2006) J. Biol. Chem. 281, 14882-14892) that (i) the p204 protein is required for the differentiation of murine P19 embryonal carcinoma stem cells to beating cardiac myocytes, and (ii) the expression of p204 in the differentiating P19 cells ... [more]

J. Biol. Chem. May. 26, 2006; 281(21);14893-906 [Pubmed: 16556596]

Throughput

  • Low Throughput

Curated By

  • BioGRID