BAIT

RFWD2

COP1, RNF200, RP11-318C24.3
ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
UPS Project
Homo sapiens
PREY

CCT3

CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5, RP11-443G18.6
chaperonin containing TCP1, subunit 3 (gamma)
GO Process (3)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Affinity purification reveals the association of WD40 protein constitutive photomorphogenic 1 with the hetero-oligomeric TCP-1 chaperonin complex in mammalian cells.

Yi C, Li S, Wang J, Wei N, Deng XW

Constitutive photomorphogenic 1 (COP1), a protein composed of a RING finger, a coiled-coil domain and seven WD40 repeats, functions as an E3 ubiquitin ligase that targets key transcription factors for ubiquitination and degradation in both higher plants and mammalian cells. While COP1 is required for light-mediated development in plants, its mammalian counterpart has been implicated in tumorigenesis. We previously showed ... [more]

Int. J. Biochem. Cell Biol. Feb. 25, 2006; 38(7);1076-83 [Pubmed: 16497536]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RFWD2 CCT3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.8957BioGRID
2259346
RFWD2 CCT3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.8289BioGRID
3054043
RFWD2 CCT3
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3570482

Curated By

  • BioGRID