A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

Dolan WP, Le AH, Schmidt H, Yuan JP, Green M, Forsburg SL

Genome stability in fission yeast requires the conserved S-phase kinase Hsk1 (Cdc7) and its partner Dfp1 (Dbf4). In addition to their established function in the initiation of DNA replication, we show that these proteins are important in maintaining genome integrity later in S phase and G2. hsk1 cells suffer increased rates of mitotic recombination and require recombination proteins for survival. ... [more]

Genetics May. 01, 2010; 185(1);39-53 [Pubmed: 20176980]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MCM2 SWI1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Matsumoto S (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SWI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]chromatin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

MCM2