BAIT

RAD51D

CG6318, Dm CG6318, Dmel\CG6318, Dmel_CG6318
CG6318 gene product from transcript CG6318-RA
GO Process (3)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Drosophila melanogaster
PREY

CG18004

Dmel\CG18004, Dmel_CG18004
CG18004 gene product from transcript CG18004-RB
GO Process (0)
GO Function (0)
GO Component (0)
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein interaction mapping: a Drosophila case study.

Formstecher E, Aresta S, Collura V, Hamburger A, Meil A, Trehin A, Reverdy C, Betin V, Maire S, Brun C, Jacq B, Arpin M, Bellaiche Y, Bellusci S, Benaroch P, Bornens M, Chanet R, Chavrier P, Delattre O, Doye V, Fehon R, Faye G, Galli T, Girault JA, Goud B, de Gunzburg J, Johannes L, Junier MP, Mirouse V, Mukherjee A, Papadopoulo D, Perez F, Plessis A, Rosse C, Saule S, Stoppa-Lyonnet D, Vincent A, White M, Legrain P, Wojcik J, Camonis J, Daviet L

The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from Drosophila melanogaster, most of them orthologous to human cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, of ... [more]

Genome Res. Mar. 01, 2005; 15(3);376-84 [Pubmed: 15710747]

Throughput

  • High Throughput

Additional Notes

  • Predicted Biological Score:D

Curated By

  • BioGRID