BAIT

CRM1

KAP124, XPO1, exportin CRM1, L000000420, YGR218W
Major karyopherin; involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin
Saccharomyces cerevisiae (S288c)
PREY

PKIA

PRKACN1
protein kinase (cAMP-dependent, catalytic) inhibitor alpha
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1.

Koyama M, Matsuura Y

The karyopherin CRM1 mediates nuclear export of proteins and ribonucleoproteins bearing a leucine-rich nuclear export signal (NES). To elucidate the precise mechanism by which NES-cargos are dissociated from CRM1 in the cytoplasm, which is important for transport directionality, we determined a 2.0-A resolution crystal structure of yeast CRM1:RanBP1:RanGTP complex, an intermediate in the disassembly of the CRM1 nuclear export complex. ... [more]

EMBO J. Jun. 16, 2010; 29(12);2002-13 [Pubmed: 20485264]

Throughput

  • Low Throughput

Additional Notes

  • Yeast Crm1 interacted with the leucine-rich nuclear export signal (NES) of human PKI alpha (FRET assay).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PKIA CRM1
Co-crystal Structure
Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Low-BioGRID
1442730

Curated By

  • BioGRID