BUD6
Gene Ontology Biological Process
- actin filament bundle assembly [IGI, IMP]
- astral microtubule organization [IMP]
- bipolar cellular bud site selection [IMP]
- budding cell apical bud growth [IGI, IMP]
- establishment of cell polarity [IMP]
- extrachromosomal circular DNA localization involved in cell aging [IMP]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- positive regulation of formin-nucleated actin cable assembly [IDA, IGI]
- pseudohyphal growth [IMP]
- regulation of protein localization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BNI1
Gene Ontology Biological Process
- actin filament bundle assembly [IGI, IMP]
- actin nucleation [IDA]
- actomyosin contractile ring actin filament bundle assembly [IMP]
- barbed-end actin filament capping [IDA]
- budding cell apical bud growth [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- formin-nucleated actin cable assembly [IDA, IGI, IMP]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- regulation of protein localization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Phenotypic Enhancement
A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole.
In Saccharomyces cerevisiae, Kar9p, one player in spindle alignment, guides the bud-ward spindle pole by linking astral microtubule plus ends to Myo2p-based transport along actin cables generated by the formins Bni1p and Bnr1p and the polarity determinant Bud6p. Initially, Kar9p labels both poles but progressively singles out the bud-ward pole. Here, we show that this polarization requires cell polarity determinants, ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: protein/peptide distribution (APO:0000209)
Additional Notes
- In bud6/bni1 mutants KAR9 association with the spindle pole is interrupted
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BNI1 BUD6 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3025 | BioGRID | 408139 | |
| BNI1 BUD6 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3728 | BioGRID | 2174061 | |
| BNI1 BUD6 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 2757929 | |
| BNI1 BUD6 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 1519617 | |
| BNI1 BUD6 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 156100 | |
| BNI1 BUD6 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low/High | - | BioGRID | 438881 | |
| BNI1 BUD6 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| BUD6 BNI1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1519620 | |
| BNI1 BUD6 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | High | - | BioGRID | 1238717 | |
| BNI1 BUD6 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| BNI1 BUD6 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| BNI1 BUD6 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 341770 | |
| BNI1 BUD6 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 1519616 | |
| BNI1 BUD6 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 450316 | |
| BNI1 BUD6 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 108840 | |
| BNI1 BUD6 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 3515533 | |
| BNI1 BUD6 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID