BAIT

OASA1

ATCYS-3A, CYTACS1, DL3480C, FCAALL.34, O-ACETHYLSERINE SULFHYDRYLASE, O-ACETYLSERINE (THIOL)LYASE, O-acetylserine (thiol) lyase (OAS-TL) isoform A1, OLD3, ONSET OF LEAF DEATH 3, AT4G14880
O-acetylserine (thiol) lyase (OAS-TL) isoform A1
Arabidopsis thaliana (Columbia)
PREY

SULTR1;2

F28K19.22, F28K19_22, SEL1, SELENATE RESISTANT 1, SULFATE TRANSPORTER, sulfate transporter 1;2, AT1G78000
sulfate transporter 1;2
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences.

Shibagaki N, Grossman AR

The sulfate ion (SO(4)(2-)) is transported into plant root cells by SO(4)(2-) transporters and then mostly reduced to sulfide (S(2-)). The S(2-) is then bonded to O-acetylserine through the activity of cysteine synthase (O-acetylserine (thiol)lyase or OASTL) to form cysteine, the first organic molecule of the SO(4)(2-) assimilation pathway. Here, we show that a root plasma membrane SO(4)(2-) transporter of ... [more]

J. Biol. Chem. Aug. 06, 2010; 285(32);25094-102 [Pubmed: 20529854]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OASA1 SULTR1;2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
SULTR1;2 OASA1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID