An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Network organization of the human autophagy system.

Behrends C, Sowa ME, Gygi SP, Harper JW

Autophagy, the process by which proteins and organelles are sequestered in autophagosomal vesicles and delivered to the lysosome/vacuole for degradation, provides a primary route for turnover of stable and defective cellular proteins. Defects in this system are linked with numerous human diseases. Although conserved protein kinase, lipid kinase and ubiquitin-like protein conjugation subnetworks controlling autophagosome formation and cargo recruitment have ... [more]

Nature Jul. 01, 2010; 466(7302);68-76 [Pubmed: 20562859]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

Exogenous expression of bait

Curated By

BioGRID

Interaction Summary

CAMKK2

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [IDA]calmodulin binding [TAS]protein tyrosine kinase activity [TAS]

Gene Ontology Cellular Component

ESYT2

Gene Ontology Molecular Function

calcium ion binding [IDA]calcium-dependent phospholipid binding [IDA]identical protein binding [IPI]phosphatidylcholine binding [IDA]phosphatidylethanolamine binding [IDA]phosphatidylinositol binding [IDA]protein binding [IPI]