An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Network organization of the human autophagy system.

Behrends C, Sowa ME, Gygi SP, Harper JW

Autophagy, the process by which proteins and organelles are sequestered in autophagosomal vesicles and delivered to the lysosome/vacuole for degradation, provides a primary route for turnover of stable and defective cellular proteins. Defects in this system are linked with numerous human diseases. Although conserved protein kinase, lipid kinase and ubiquitin-like protein conjugation subnetworks controlling autophagosome formation and cargo recruitment have ... [more]

Nature Jul. 01, 2010; 466(7302);68-76 [Pubmed: 20562859]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

Exogenous expression of bait

Curated By

BioGRID

Interaction Summary

GABARAP

Gene Ontology Biological Process

Gene Ontology Molecular Function

GABA receptor binding [IPI]beta-tubulin binding [IDA]microtubule binding [IBA]protein binding [IPI]

Gene Ontology Cellular Component

IPO5

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase inhibitor activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein transporter activity [ISS]