BAIT

AT1G19750

F14P1.15, F14P1_15
UV-B response protein-like protein CSAat1b
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

DDB1A

C6L9.100, C6L9_100, damaged DNA binding protein 1A, AT4G05420
DNA damage-binding protein 1a
GO Process (2)
GO Function (2)
GO Component (3)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Arabidopsis cockayne syndrome A-like proteins 1A and 1B form a complex with CULLIN4 and damage DNA binding protein 1A and regulate the response to UV irradiation.

Zhang C, Guo H, Zhang J, Guo G, Schumaker KS, Guo Y

In plants, as in animals, DNA is constantly subject to chemical modification. UV-B irradiation is a major genotoxic agent and has significant effects on plant growth and development. Through forward genetic screening, we identified a UV-B-sensitive mutant (csaat1a-3) in Arabidopsis thaliana, in which expression of CSAat1A, encoding a Cockayne Syndrome A-like protein, is reduced due to insertion of a T-DNA ... [more]

Plant Cell Jul. 01, 2010; 22(7);2353-69 [Pubmed: 20622147]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AT1G19750 DDB1A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID