Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The ZNF217 oncogene is a candidate organizer of repressive histone modifiers.

Banck MS, Li S, Nishio H, Wang C, Beutler AS, Walsh MJ

The zinc finger protein 217 (ZNF217) is an important oncogene based on the high frequency of amplification and overexpression in many cancer types, but its molecular mode of gene regulation is poorly understood. We purified a complex of nuclear ZNF217-binding proteins by affinity chromatography and identified its components by mass spectrometry as Jarid1b/Plu-1, G9a, LSD1, CoREST and CtBP1. Individual binding ... [more]

Epigenetics Feb. 16, 2009; 4(2);100-6 [Pubmed: 19242095]

Throughput

  • Low Throughput

Curated By

  • BioGRID