PREY

TAF2

CIF150, MRT40, TAF2B, TAFII150
TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function.

Knuesel MT, Meyer KD, Bernecky C, Taatjes DJ

The human CDK8 subcomplex (CDK8, cyclin C, Med12, and Med13) negatively regulates transcription in ways not completely defined; past studies suggested CDK8 kinase activity was required for its repressive function. Using a reconstituted transcription system together with recombinant or endogenous CDK8 subcomplexes, we demonstrate that, in fact, Med12 and Med13 are critical for subcomplex-dependent repression, whereas CDK8 kinase activity is ... [more]

Genes Dev. Feb. 15, 2009; 23(4);439-51 [Pubmed: 19240132]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TBP TAF2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
TBP TAF2
Co-purification
Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Low-BioGRID
-

Curated By

  • BioGRID