An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing.

Zhao Q, Rank G, Tan YT, Li H, Moritz RL, Simpson RJ, Cerruti L, Curtis DJ, Patel DJ, Allis CD, Cunningham JM, Jane SM

Mammalian gene silencing is established through methylation of histones and DNA, although the order in which these modifications occur remains contentious. Using the human beta-globin locus as a model, we demonstrate that symmetric methylation of histone H4 arginine 3 (H4R3me2s) by the protein arginine methyltransferase PRMT5 is required for subsequent DNA methylation. H4R3me2s serves as a direct binding target for ... [more]

Nat. Struct. Mol. Biol. Mar. 01, 2009; 16(3);304-11 [Pubmed: 19234465]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PRMT5

Gene Ontology Biological Process

Gene Ontology Molecular Function

core promoter sequence-specific DNA binding [ISS]histone-arginine N-methyltransferase activity [IBA]methyltransferase activity [IDA]protein binding [IPI]protein-arginine omega-N symmetric methyltransferase activity [IMP]ribonucleoprotein complex binding [IPI]transcription corepressor activity [ISS]

Gene Ontology Cellular Component

DNMT3A