A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology.

Guan XL, Souza CM, Pichler H, Dewhurst G, Schaad O, Kajiwara K, Wakabayashi H, Ivanova T, Castillon GA, Piccolis M, Abe F, Loewith R, Funato K, Wenk MR, Riezman H

Sterols and sphingolipids are limited to eukaryotic cells, and their interaction has been proposed to favor formation of lipid microdomains. Although there is abundant biophysical evidence demonstrating their interaction in simple systems, convincing evidence is lacking to show that they function together in cells. Using lipid analysis by mass spectrometry and a genetic approach on mutants in sterol metabolism, we ... [more]

Mol. Biol. Cell Apr. 01, 2009; 20(7);2083-95 [Pubmed: 19225153]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ERG4 SUR2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1884	BioGRID	2113838

Curated By

BioGRID

Interaction Summary

ERG4

Gene Ontology Biological Process

Gene Ontology Molecular Function

delta24(24-1) sterol reductase activity [IDA]

Gene Ontology Cellular Component

SUR2