BAIT

LIP1

sphingosine N-acyltransferase subunit LIP1, YMR298W

Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis

GO Process (1)

GO Function (1)

GO Component (4)

Gene Ontology Biological Process

ceramide biosynthetic process [IMP, IPI]

Gene Ontology Molecular Function

sphingosine N-acyltransferase activity [IMP]

Gene Ontology Cellular Component

acyl-CoA ceramide synthase complex [IDA]

endoplasmic reticulum [IDA]

endoplasmic reticulum membrane [IDA]

nuclear envelope [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ELO3

SUR4, APA1, SRE1, VBM1, fatty acid elongase ELO3, L000002245, YLR372W

Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Defect of synthesis of very long-chain fatty acids confers resistance to growth inhibition by inositol phosphorylceramide synthase repression in yeast Saccharomyces cerevisiae.

Tani M, Kuge O

Aureobasidin A (AbA) inhibits Aur1p, an enzyme catalyzing the formation of inositol phosphorylceramide in the yeast Saccharomyces cerevisiae. AbA treatment results not only in reductions in complex sphingolipid levels but also in accumulation of ceramides, both of which are believed to lead to the growth defect caused by this inhibitor. We screened for mutants showing resistance to this drug, and ... [more]

Unknown Aug. 12, 2010; 0(0); [Pubmed: 20709688]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Additional Notes

Deletion of ELO3 causes an enhanced growth defect when LIP1 is overexpressed

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LIP1 ELO3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3899	BioGRID	2008093
ELO3 LIP1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Hoppins S (2011)	High	-9.9004	BioGRID	586639
LIP1 ELO3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Surma MA (2013)	High	-17.9851	BioGRID	894902

Curated By

BioGRID

Interaction Summary