Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ccq1p and the Condensin Proteins Cut3p and Cut14p Prevent Telomere Entanglements in the Fission Yeast Schizosaccharomyces pombe.

Motwani T, Doris R, Holmes SG, Flory MR

The S. pombe telomere-associated protein Ccq1p has previously been shown to participate in telomerase recruitment, heterochromatin formation, and suppression of checkpoint activation. Here we characterize a critical role for Ccq1p in mitotic transit. We show that mitotic cells lacking Ccq1p lose minichromosomes at high frequencies, while conditional knockdown of Ccq1p expression results in telomere bridging within one cell cycle. Elevating ... [more]

Unknown Aug. 13, 2010; 0(0); [Pubmed: 20709788]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUT3 CCQ1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.8874BioGRID
791077
CCQ1 CUT3
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
450009

Curated By

  • BioGRID