BAIT

SRS2

HPR5, DNA helicase SRS2, RADH1, RADH, L000000809, L000001578, YJL092W

DNA helicase and DNA-dependent ATPase; involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; functional homolog of human RTEL1

GO Process (6)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

DNA duplex unwinding [IDA]

DNA repair [IGI]

double-strand break repair via nonhomologous end joining [IDA]

negative regulation of DNA recombination [IMP]

negative regulation of double-strand break repair via homologous recombination [IDA]

protein-DNA complex disassembly [IDA, IMP]

Gene Ontology Molecular Function

DNA helicase activity [IDA, ISS]

Gene Ontology Cellular Component

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ESC2

L000004423, YDR363W

Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member

UPS Project

GO Process (5)

GO Function (0)

GO Component (1)

Gene Ontology Biological Process

chromatin silencing at silent mating-type cassette [IMP]

double-strand break repair via homologous recombination [IGI, IMP]

intra-S DNA damage checkpoint [IGI, IMP]

mitotic sister chromatid cohesion [IGI, IMP]

positive regulation of protein sumoylation [IMP]

Gene Ontology Cellular Component

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Global mapping of the yeast genetic interaction network.

Tong AH, Lesage G, Bader GD, Ding H, Xu H, Xin X, Young J, Berriz GF, Brost RL, Chang M, Chen Y, Cheng X, Chua G, Friesen H, Goldberg DS, Haynes J, Humphries C, He G, Hussein S, Ke L, Krogan N, Li Z, Levinson JN, Lu H, Menard P, Munyana C, Parsons AB, Ryan O, Tonikian R, Roberts T, Sdicu AM, Shapiro J, Sheikh B, Suter B, Wong SL, Zhang LV, Zhu H, Burd CG, Munro S, Sander C, Rine J, Greenblatt J, Peter M, Bretscher A, Bell G, Roth FP, Brown GW, Andrews B, Bussey H, Boone C

A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of ... [more]

Science Feb. 06, 2004; 303(5659);808-13 [Pubmed: 14764870]

Throughput

High Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRS2 ESC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-10.3091	BioGRID	214172
SRS2 ESC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.5878	BioGRID	390693
ESC2 SRS2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.5878	BioGRID	369972
SRS2 ESC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2945	BioGRID	2135883
SRS2 ESC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Kuzmin E (2018)	High	-0.377	BioGRID	2438250
ESC2 SRS2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Urulangodi M (2015)	Low	-	BioGRID	1519369
SRS2 ESC2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Urulangodi M (2015)	Low	-	BioGRID	1519370
ESC2 SRS2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Decourty L (2008)	High	-	BioGRID	335699
ESC2 SRS2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Jorgensen SW (2019)	Low	-	BioGRID	2650271
SRS2 ESC2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Pan X (2006)	High	-	BioGRID	453993
ESC2 SRS2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Tong AH (2004)	High	-	BioGRID	451660
SRS2 ESC2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Xu H (2004)	Low	-	BioGRID	165982
ESC2 SRS2	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Urulangodi M (2015)	Low	-	BioGRID	1519375
SRS2 ESC2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Urulangodi M (2015)	Low	-	BioGRID	1519371

Curated By

BioGRID

Interaction Summary

SRS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA helicase activity [IDA, ISS]

Gene Ontology Cellular Component

ESC2

Gene Ontology Biological Process

Gene Ontology Cellular Component

Synthetic Growth Defect

Publication

Global mapping of the yeast genetic interaction network.

Throughput

Ontology Terms

Related interactions

Curated By