BAIT
RAD6
PSO8, UBC2, E2 ubiquitin-conjugating protein RAD6, L000001560, YGL058W
Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, as well as endoplasmic reticulum-associated protein degradation (ERAD) with Ubr1p in the absence of canonical ER membrane ligases
GO Process (17)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- ER-associated ubiquitin-dependent protein catabolic process [IGI]
- chromatin silencing at telomere [IMP]
- double-strand break repair via homologous recombination [IGI]
- error-free postreplication DNA repair [IGI]
- error-free translesion synthesis [IGI]
- error-prone translesion synthesis [IGI]
- histone monoubiquitination [IMP]
- meiotic DNA double-strand break formation [IMP]
- mitotic G1 DNA damage checkpoint [IMP]
- protein monoubiquitination [IMP]
- protein polyubiquitination [IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of dipeptide transport [IMP]
- telomere maintenance via recombination [IGI]
- transcription from RNA polymerase II promoter [IPI]
- ubiquitin-dependent protein catabolic process via the N-end rule pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
FKH2
forkhead family transcription factor FKH2, L000002608, YNL068C
Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; facilitates clustering and activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; relocalizes to the cytosol in response to hypoxia; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
GO Process (10)
GO Function (8)
GO Component (4)
Gene Ontology Biological Process
- chromatin remodeling [IGI, IMP]
- mitochondrion organization [IBA]
- negative regulation of chromatin silencing at silent mating-type cassette [IGI, IMP]
- negative regulation of pseudohyphal growth [IGI, IMP]
- negative regulation of transcription involved in G1/S transition of mitotic cell cycle [IGI]
- negative regulation of transcription involved in G2/M transition of mitotic cell cycle [IGI]
- positive regulation of DNA-dependent DNA replication initiation [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription involved in G2/M transition of mitotic cell cycle [IGI, IMP]
- regulation of sequence-specific DNA binding transcription factor activity [IBA]
Gene Ontology Molecular Function- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA]
- RNA polymerase II transcription factor binding [IDA, IMP]
- RNA polymerase II transcription factor binding transcription factor activity [IDA, IGI, IMP]
- double-stranded DNA binding [IBA]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IBA]
- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA]
- RNA polymerase II transcription factor binding [IDA, IMP]
- RNA polymerase II transcription factor binding transcription factor activity [IDA, IGI, IMP]
- double-stranded DNA binding [IBA]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IBA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
A DNA integrity network in the yeast Saccharomyces cerevisiae.
A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, 16 functional modules or minipathways were defined based on patterns of global SFL interactions. Modules or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative stress response were identified as the major guardians ... [more]
Cell Mar. 10, 2006; 124(5);1069-81 [Pubmed: 16487579]
Throughput
- High Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- confirmed by tetrad analysis
Curated By
- BioGRID