MUS81
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RAD54
Gene Ontology Biological Process
- DNA geometric change [IDA]
- chromatin remodeling [IDA]
- double-strand break repair via single-strand annealing [IMP]
- double-strand break repair via synthesis-dependent strand annealing [TAS]
- heteroduplex formation [IDA]
- positive regulation of endodeoxyribonuclease activity [IDA]
- telomere maintenance via recombination [IMP]
Gene Ontology Molecular Function
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
A DNA integrity network in the yeast Saccharomyces cerevisiae.
A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, 16 functional modules or minipathways were defined based on patterns of global SFL interactions. Modules or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative stress response were identified as the major guardians ... [more]
Throughput
- High Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- confirmed by tetrad analysis
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RAD54 MUS81 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
MUS81 RAD54 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.0831 | BioGRID | 2605088 | |
MUS81 RAD54 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.106 | BioGRID | 2605151 | |
RAD54 MUS81 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 345768 | |
RAD54 MUS81 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 457591 | |
RAD54 MUS81 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID