BAIT

BRIZ1

BRAP2 RING ZnF UBP domain-containing protein 1, T24P15.7, T24P15_7, AT2G42160
RING domain containing protein BRIZ1
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

BRIZ2

BRAP2 RING ZnF UBP domain-containing protein 2, T19L18.19, T19L18_19, AT2G26000
RING domain containing protein BRIZ2
GO Process (1)
GO Function (4)
GO Component (1)
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

BRIZ1 and BRIZ2 proteins form a heteromeric E3 ligase complex required for seed germination and post-germination growth in Arabidopsis thaliana.

Hsia MM, Callis J

Ubiquitin pathway E3 ligases are an important component conferring specificity and regulation in ubiquitin attachment to substrate proteins. The Arabidopsis thaliana RING (Really Interesting New Gene) domain-containing proteins BRIZ1 and BRIZ2 are essential for normal seed germination and post-germination growth. Loss of either BRIZ1 (At2g42160) or BRIZ2 (At2g26000) results in a severe phenotype. Heterozygous parents produce progeny that segregate 3:1 ... [more]

Unknown Sep. 01, 2010; 0(0); [Pubmed: 20810661]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BRIZ1 BRIZ2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
BRIZ2 BRIZ1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID