BAIT

POL30

proliferating cell nuclear antigen, PCNA, L000001463, YBR088C
Proliferating cell nuclear antigen (PCNA); functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
Saccharomyces cerevisiae (S288c)
PREY

SAS4

L000004214, YDR181C
Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
GO Process (1)
GO Function (2)
GO Component (3)
Saccharomyces cerevisiae (S288c)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Proliferating cell nuclear antigen (PCNA) is required for cell-cycle regulated silent chromatin on replicated and nonreplicated genes.

Miller A, Chen J, Takasuka TE, Jacobi JL, Kaufman PD, Irudayaraj JM, Kirchmaier AL

In Saccharomyces cerevisiae, silent chromatin is formed at HMR upon the passage through S phase, yet neither the initiation of DNA replication at silencers nor the passage of a replication fork through HMR are required for silencing. Paradoxically, mutations in the DNA replication processivity factor, POL30, disrupt silencing despite this lack of requirement for DNA replication in the establishment of ... [more]

Unknown Sep. 02, 2010; 0(0); [Pubmed: 20813847]

Throughput

  • Low Throughput

Curated By

  • BioGRID