BAIT

STE24

AFC1, PIO2, L000003457, YJR117W

Highly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans

GO Process (2)

GO Function (1)

GO Component (4)

Gene Ontology Biological Process

CAAX-box protein processing [IMP]

peptide pheromone maturation [IDA, IMP]

Gene Ontology Molecular Function

metalloendopeptidase activity [IDA, IMP, ISS]

Gene Ontology Cellular Component

integral component of endoplasmic reticulum membrane [IDA]

integral component of membrane [ISM]

mitochondrial outer membrane [IDA]

nuclear inner membrane [IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ELO3

SUR4, APA1, SRE1, VBM1, fatty acid elongase ELO3, L000002245, YLR372W

Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum.

Jonikas MC, Collins SR, Denic V, Oh E, Quan EM, Schmid V, Weibezahn J, Schwappach B, Walter P, Weissman JS, Schuldiner M

Protein folding in the endoplasmic reticulum is a complex process whose malfunction is implicated in disease and aging. By using the cell's endogenous sensor (the unfolded protein response), we identified several hundred yeast genes with roles in endoplasmic reticulum folding and systematically characterized their functional interdependencies by measuring unfolded protein response levels in double mutants. This strategy revealed multiple conserved ... [more]

Science Mar. 27, 2009; 323(5922);1693-7 [Pubmed: 19325107]

Throughput

High Throughput

Ontology Terms

phenotype: protein/peptide accumulation (APO:0000149)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STE24 ELO3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	3	BioGRID	3616720
STE24 ELO3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Hoppins S (2011)	High	-2.5189	BioGRID	585446
ELO3 STE24	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Hoppins S (2011)	High	-2.5189	BioGRID	583920
ELO3 STE24	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Jonikas MC (2009)	High	-	BioGRID	460796

Curated By

BioGRID

Interaction Summary