BAIT

ATF7IP

AM, ATF-IP, MCAF, MCAF1, p621, RP11-502N13.4
activating transcription factor 7 interacting protein
Homo sapiens
PREY

ERCC2

COFS2, EM9, TFIIH, TTD, XPD
excision repair cross-complementation group 2
GO Process (28)
GO Function (7)
GO Component (7)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity.

Liu L, Ishihara K, Ichimura T, Fujita N, Hino S, Tomita S, Watanabe S, Saitoh N, Ito T, Nakao M

Telomerase maintains telomere length and is implicated in senescence and immortalization of mammalian cells. Two essential components for this enzyme are telomerase reverse transcriptase (TERT) and the telomerase RNA component (encoded by the TERC gene). These telomerase subunit genes are known to be mainly expressed by specificity protein 1 (Sp1). MBD1-containing chromatin-associated factor 1 (MCAF1), also known as ATFa-associated modulator ... [more]

J. Biol. Chem. Feb. 20, 2009; 284(8);5165-74 [Pubmed: 19106100]

Throughput

  • Low Throughput

Curated By

  • BioGRID