VPS16
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLK2
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO, ISS]
- Rap protein signal transduction [IDA]
- Ras protein signal transduction [IDA]
- long term synaptic depression [IDA]
- long-term synaptic potentiation [IDA]
- memory [ISO, ISS]
- mitotic cell cycle [ISO]
- mitotic cell cycle checkpoint [ISO, ISS]
- mitotic spindle organization [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of dendritic spine development [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of protein binding [IDA]
- protein phosphorylation [IDA, ISO]
- regulation of centriole replication [ISO, ISS]
- regulation of synaptic plasticity [IDA, IMP, ISO, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- centriole [ISO, ISS]
- centrosome [ISO, ISS]
- dendrite [IDA]
- intracellular [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ubiquitylation and degradation of serum-inducible kinase by hVPS18, a RING-H2 type ubiquitin ligase.
Serum-inducible kinase (SNK) is a member of polo-like kinases that serve as regulators of multiple events during cell division. Rapid changes in the activity and abundance of SNK were reported after the serum stimulation and after the activation of synaptic transmission in the brain. Yet the detailed mechanisms that control the level of SNK protein have not been fully elucidated. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PLK2 VPS16 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PLK2 VPS16 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID