BAIT

MDM2

ACTFS, HDMX, hdm2
MDM2 proto-oncogene, E3 ubiquitin protein ligase
GO Process (25)
GO Function (7)
GO Component (9)
Homo sapiens

Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

Concerted activation of the Mdm2 promoter by p72 RNA helicase and the coactivators p300 and P/CAF.

Shin S, Janknecht R

A scarcely studied and under-recognized feature of RNA helicases is their ability to regulate gene transcription. In particular, very little is known about the role of p72 RNA helicase in gene regulation. Here, we have analyzed how this helicase may enhance promoter activity. We demonstrate that p72 RNA helicase forms complexes with the homologous coactivators p300 and CBP in vitro ... [more]

J. Cell. Biochem. Aug. 01, 2007; 101(5);1252-65 [Pubmed: 17226766]

Throughput

  • Low Throughput

Curated By

  • BioGRID