PREY

KAT2B

CAF, P/CAF, PCAF
K(lysine) acetyltransferase 2B
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Concerted activation of the Mdm2 promoter by p72 RNA helicase and the coactivators p300 and P/CAF.

Shin S, Janknecht R

A scarcely studied and under-recognized feature of RNA helicases is their ability to regulate gene transcription. In particular, very little is known about the role of p72 RNA helicase in gene regulation. Here, we have analyzed how this helicase may enhance promoter activity. We demonstrate that p72 RNA helicase forms complexes with the homologous coactivators p300 and CBP in vitro ... [more]

J. Cell. Biochem. Aug. 01, 2007; 101(5);1252-65 [Pubmed: 17226766]

Throughput

  • Low Throughput

Curated By

  • BioGRID