A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development.

Duan Q, Kita D, Li C, Cheung AY, Wu HM

Plant RHO GTPases (RAC/ROPs) mediate multiple extracellular signals ranging from hormone to stress and regulate diverse cellular processes important for polarized cell growth, differentiation, development, reproduction, and responses to the environment. They shuttle between the GDP-bound inactive state and the GTP-bound activated state and their activation is predominantly mediated by a family of guanine nucleotide exchange factors (GEFs) referred to ... [more]

Unknown Sep. 27, 2010; 0(0); [Pubmed: 20876100]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FER ROPGEF1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Duan Q (2010)	Low	-	BioGRID	-
ROPGEF1 FER	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Duan Q (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ROPGEF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rho guanyl-nucleotide exchange factor activity [IDA]

Gene Ontology Cellular Component

FER