Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Calcium signalling in T cells stimulated by a cyclophilin B-binding protein.

Bram RJ, Crabtree GR

The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin, cyclosporin A binds and inactivates the key signalling intermediate calcineurin. To identify potential cellular homologues of cyclosporin A that might regulate calcium signalling, we have cloned human genes encoding cyclophilin B-binding-proteins using the yeast two-hybrid system. ... [more]

Nature Sep. 22, 1994; 371(6495);355-8 [Pubmed: 7522304]

Throughput

  • Low Throughput

Curated By

  • BioGRID