BAIT

TRIM63

IRF, MURF1, MURF2, RNF28, SMRZ
tripartite motif containing 63, E3 ubiquitin protein ligase
GO Process (2)
GO Function (3)
GO Component (2)
Homo sapiens
PREY

AKR7A2

AFAR, AFAR1, AFB1-AR1, AKR7
aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

MURF-1 and MURF-2 target a specific subset of myofibrillar proteins redundantly: towards understanding MURF-dependent muscle ubiquitination.

Witt SH, Granzier H, Witt CC, Labeit S

MURF-1, MURF-2 and MURF-3 are a specific class of RING finger proteins that are expressed in striated muscle tissues. MURF-1 has been suggested to act as an ubiquitin ligase, thereby controlling proteasome-dependent degradation of muscle proteins. Here, we performed yeast two-hybrid (YTH) screens of skeletal muscle cDNA libraries with MURF-1 baits to identify potential myocellular targets of MURF-1-dependent ubiquitination. This ... [more]

J. Mol. Biol. Jul. 22, 2005; 350(4);713-22 [Pubmed: 15967462]

Throughput

  • Low Throughput

Curated By

  • BioGRID