BAIT

DAF-2

CELE_Y55D5A.5
Protein DAF-2
Caenorhabditis elegans
PREY

CPB-3

CELE_B0414.5, B0414.5
cpb-3 encodes a CPEB orthologous to Drosophila ORB, zebrafish ZORBA, and human CPEB1, and paralogous to FOG-1 and CPB-1/2; CPB-3 inhibits physiological apoptosis in oocytes, keeping it down to a normal level of ~50% in hermaphrodite gonads; independently of apoptosis, CPB-3 is also required for normal oogenesis, sperm/oocyte switching (upstream of FEM-3), and the transistion from mitosis to meiosis by oocytes (in parallel with GLD-3); cpb-3(RNAi) results in elevated (though not 100%) physiological germ cell apoptosis, independent of CEP-1; in cpb-3(RNAi) animals, excess physiological apoptosis actually promotes fertility, since cpb-3(RNAi) hermaphrodites have larger brood sizes than those with apoptosis suppressed by ced-3(n717), and aging ced-3(n717);cpb-3(RNAi) animals have abnormal oocytes that fail to exit pachytene and proceed through diakinesis; CPB-3 is mainly cytoplasmic, and strongly expressed in pachytene oocytes; cpb-3(bt17);daz-1(tj3) hermaphrodites have a synthetic phenotype of total sterility and masculinization; CPB-3 colocalizes with DAZ-1 in vivo, coimmunoprecipitates with DAZ-1, and binds DAZ-1 in two-hybrid assays.
GO Process (3)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Caenorhabditis elegans

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global analysis of genetic interactions in Caenorhabditis elegans.

Byrne AB, Weirauch MT, Wong V, Koeva M, Dixon SJ, Stuart JM, Roy PJ

BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]

J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]

Quantitative Score

  • 1.8571 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: organism development variant (WBPHENOTYPE:0000531)

Additional Notes

  • A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
  • Negative Genetic

Curated By

  • BioGRID