BAIT
BAR-1
CELE_C54D1.6, pvl-1, spy-1, C54D1.6
bar-1 encodes a beta-catenin; during C. elegans development, BAR-1 likely functions as a transcriptional coactivator whose activity is required for Q neuroblast migration, P12 cell fate specification, and P3.p through P8.p vulval cell fate specification at two different stages of development; in specifying vulval cell fates, bar-1 interacts with Wnt and MAPK signaling pathways to regulate proper expression of the LIN-39 homeodomain transcription factor, overexpresion of which can partially rescue the bar-1 mutant phenotype; in yeast two-hybrid assays, BAR-1 interacts strongly with the POP-1/TCF transcription factor, and when fused to the Gal4 DNA binding domain, BAR-1 can function in yeast as a transcriptional coactivator; during larval development, BAR-1 expression begins in P3.p through P8.p at the late L1 stage and then disappears from these cells by the mid-L3 stage; BAR-1 is also expressed in P12, in the seam cells, and in cells of the somatic gonad; BAR-1 subcellular localization, assessed using an integrated transgene, reveals localization to the cytoplasm, nucleus, and cell junctions; genetic mosaic analyses indicate that, in P4.p and in P12, bar-1 acts cell autonomously to specify cell fates.
GO Process (16)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- Ras protein signal transduction [IGI]
- Wnt signaling pathway [IMP]
- cell adhesion [TAS]
- cell fate specification [IMP]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- mating behavior [IMP]
- morphogenesis of an epithelium [IMP]
- oviposition [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of vulval development [IMP]
- receptor-mediated endocytosis [IMP]
- regulation of backward locomotion [IMP]
- regulation of cell fate specification [IMP]
- regulation of cell migration [IMP]
- regulation of vulval development [IGI, IMP]
Gene Ontology Molecular Function
Caenorhabditis elegans
PREY
LIN-35
CELE_C32F10.2, C32F10.2
lin-35 encodes the C. elegans retinoblastoma protein (Rb) ortholog; lin-35 was first identified in screens for synthetic multivulva (synMuv) genes and as a class B synMuv gene, functions redundantly with class A genes to antagonize Ras signaling and negatively regulate vulval development; in addition, loss of lin-35 activity results in enhanced RNA interference; lin-35 activity is also required redundantly with: 1) pha-1 and ubc-18 for early steps in pharyngeal morphogenesis, 2) fzr-1 for normal patterns of postembryonic proliferation, 3) xnp-1 for somatic gonad development, and 4) psa-1 for fertility and embryonic and larval development; on its own, lin-35 is also required for wild-type levels of fertility; LIN-35 is expressed broadly in embryos and L1 larvae, but in later larvae and adults is detected in vulval precursor cells and their descendants as well as a subset of head and tail cells.
GO Process (11)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- apoptotic process [IMP]
- body morphogenesis [IMP]
- embryo development ending in birth or egg hatching [IGI, IMP]
- embryonic digestive tract morphogenesis [IGI]
- mitotic cell cycle arrest [IGI]
- negative regulation of transcription from RNA polymerase I promoter [ISS]
- negative regulation of vulval development [IGI, IMP]
- nematode larval development [IGI, IMP]
- oviposition [IMP]
- positive regulation of multicellular organism growth [IGI]
- reproduction [IGI, IMP]
Gene Ontology Molecular Function
Caenorhabditis elegans
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global analysis of genetic interactions in Caenorhabditis elegans.
BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]
J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]
Quantitative Score
- 4.2857 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: organism development variant (WBPHENOTYPE:0000531)
Additional Notes
- A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
- Negative Genetic
Curated By
- BioGRID