BAIT
SOS-1
CELE_T28F12.3, let-341, T28F12.3
sos-1 encodes an ortholog of Son of sevenless, a guanine nucleotide exchange factor; sos-1 is required for viability, sex myoblast migration, vulval induction, and oogenesis; sos-1 acts genetically downstream of let-23 and upstream of let-60 with respect to vulval development; SOS-1 binds bovine calmodulin in vitro in a calcium-dependent manner.
GO Process (10)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
- body morphogenesis [IMP]
- cell migration [IMP]
- embryo development [IMP]
- embryo development ending in birth or egg hatching [IMP]
- female gamete generation [IMP]
- oviposition [IMP]
- positive regulation of Ras GTPase activity [ISS]
- positive regulation of vulval development [IGI]
- reproduction [IMP]
- secretion by cell [IMP]
Gene Ontology Molecular Function
Caenorhabditis elegans
PREY
ZYG-9
CELE_F22B5.7, F22B5.7
zyg-9 encodes a predicted microtubule-association protein (MAP) of the XMAP215 family; during early embryonic development, maternal zyg-9 activity is required for microtubule-dependent processes such as meiotic and mitotic spindle organization and pronuclear migration; further, zyg-9 is essential for formation of long astral microtubules; ZYG-9 is required for centrosomal localization of TAC-1, the sole C. elegans TACC family member, with which it interacts, and mutually stabilizes, in vivo; in early embryos, ZYG-9 localizes to the meiotic spindle and spindle poles, as well as to mitotic centrosomes; during metaphase and anaphase ZYG-9 localizes to the central spindle region, and during interphase ZYG-9 is cytoplasmic; proper localization of ZYG-9 to the meiotic spindle is dependent upon wild-type activity of MEI-1, an ATPase essential for meiotic spindle formation, while proper localization of ZYG-9 to centrosomes is dependent, at least in part, upon TAC-1.
GO Process (14)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- body morphogenesis [IMP]
- cell division [IMP]
- centrosomal and pronuclear rotation [IMP]
- embryo development [IEP]
- embryo development ending in birth or egg hatching [IMP]
- hermaphrodite genitalia development [IMP]
- meiotic spindle organization [IMP]
- microtubule polymerization [IMP]
- microtubule-based process [IMP]
- mitotic spindle organization [IMP]
- oviposition [IMP]
- pronuclear migration [IMP]
- spindle elongation [IMP]
- spindle organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global analysis of genetic interactions in Caenorhabditis elegans.
BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]
J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]
Quantitative Score
- 1.5 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: organism development variant (WBPHENOTYPE:0000531)
Additional Notes
- A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
- Negative Genetic
Curated By
- BioGRID