BAIT

BAR-1

CELE_C54D1.6, pvl-1, spy-1, C54D1.6
bar-1 encodes a beta-catenin; during C. elegans development, BAR-1 likely functions as a transcriptional coactivator whose activity is required for Q neuroblast migration, P12 cell fate specification, and P3.p through P8.p vulval cell fate specification at two different stages of development; in specifying vulval cell fates, bar-1 interacts with Wnt and MAPK signaling pathways to regulate proper expression of the LIN-39 homeodomain transcription factor, overexpresion of which can partially rescue the bar-1 mutant phenotype; in yeast two-hybrid assays, BAR-1 interacts strongly with the POP-1/TCF transcription factor, and when fused to the Gal4 DNA binding domain, BAR-1 can function in yeast as a transcriptional coactivator; during larval development, BAR-1 expression begins in P3.p through P8.p at the late L1 stage and then disappears from these cells by the mid-L3 stage; BAR-1 is also expressed in P12, in the seam cells, and in cells of the somatic gonad; BAR-1 subcellular localization, assessed using an integrated transgene, reveals localization to the cytoplasm, nucleus, and cell junctions; genetic mosaic analyses indicate that, in P4.p and in P12, bar-1 acts cell autonomously to specify cell fates.
Caenorhabditis elegans
PREY

POP-1

CELE_W10C8.2, sys-2, W10C8.2
pop-1 encodes an HMG box-containing protein that is the sole C. elegans member of the TCF/LEF family of transcription factors; in C. elegans, POP-1 functions as a component of the canonical and noncanonical Wnt signaling pathways that are required for cell migrations and binary cell fate decisions associated with asymmetric cell division, respectively; in yeast two-hybrid assays, the POP-1 N-terminal beta-catenin binding domain interacts with BAR-1/beta-catenin as well as with the more divergent beta-catenin, SYS-1; when coexpressed with SYS-1, POP-1 is able to activate transcription from a promoter with TCF binding sites; during development, maternally provided POP-1 is first detected in the nuclei of maturing oocytes and then in nearly all cells of the early embryo; in sister blastomeres in the early embryo, POP-1 is detected at lower levels in posterior blastomeres, such as E and P3, than in corresponding anterior blastomeres, MS and C; in later developmental stages, POP-1 is detected in a subset of tissues including hypodermal seam cells, gonadal precursors, and the developing vulva; in the vulva, POP-1 also exhibits an asymmetric staining pattern, with sister cells showing high or low levels of POP-1 depending upon their orientation along the anterior/posterior axis of the vulva.
Caenorhabditis elegans

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global analysis of genetic interactions in Caenorhabditis elegans.

Byrne AB, Weirauch MT, Wong V, Koeva M, Dixon SJ, Stuart JM, Roy PJ

BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]

J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]

Quantitative Score

  • 4.875 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: organism development variant (WBPHENOTYPE:0000531)

Additional Notes

  • A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
  • Negative Genetic

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BAR-1 POP-1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-WormBase
-
POP-1 BAR-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
POP-1 BAR-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2611346
BAR-1 POP-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-WormBase
-
POP-1 BAR-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID