BAIT
GLP-1
CELE_F02A9.6, emb-33, F02A9.6
glp-1 encodes an N-glycosylated transmembrane protein that, along with LIN-12, comprises one of two C. elegans members of the LIN-12/Notch family of receptors; from the N- to the C-terminus, GLP-1 is characterized by ten extracellular EGF-like repeats, three LIN-12/Notch repeats, a CC-linker, a transmembrane domain, a RAM domain, six intracellular ankyrin repeats, and a PEST sequence; in C. elegans, GLP-1 activity is required for cell fate specification in germline and somatic tissues; in the germline, GLP-1, acting as a receptor for the DSL family ligand LAG-2, is essential for mitotic proliferation of germ cells and maintenance of germline stem cells; in somatic tissues, maternally provided GLP-1, acting as a receptor for the DSL family ligand APX-1, is required for inductive interactions that specify the fates of certain embryonic blastomeres; GLP-1 is also required for some later embryonic cell fate decisions, and in these decisions its activity is functionally redundant with that of LIN-12; GLP-1 expression is regulated temporally and spatially via translational control, as GLP-1 mRNA, present ubiquitously in the germline and embryo, yields detectable protein solely in lateral, interior, and endomembranes of distal, mitotic germ cells, and then predominantly in the AB blastomere and its descendants in the early embryo; proper spatial translation of glp-1 mRNA in the embryo is dependent upon genes such as the par genes, that are required for normal anterior-posterior asymmetry in the early embryo; signaling through GLP-1 controls the activity of the downstream Notch pathway components LAG-3 and LAG-1, the latter being predicted to function as part of a transcriptional feedback mechanism that positively regulates GLP-1 expression; signaling through the DNA-binding protein LAG-1 is believed to involve a direct interaction between LAG-1 and the GLP-1 RAM and ankyrin domains
GO Process (11)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- body morphogenesis [IMP]
- cell fate specification [IGI]
- determination of adult lifespan [IMP]
- embryo development ending in birth or egg hatching [IMP]
- embryonic pattern specification [IGI, IMP]
- maintenance of dauer [IGI]
- nematode larval development [IGI, IMP]
- pharyngeal muscle development [IGI, IMP]
- regulation of cell proliferation [IMP]
- regulation of meiosis [IMP]
- reproduction [IMP]
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
POP-1
CELE_W10C8.2, sys-2, W10C8.2
pop-1 encodes an HMG box-containing protein that is the sole C. elegans member of the TCF/LEF family of transcription factors; in C. elegans, POP-1 functions as a component of the canonical and noncanonical Wnt signaling pathways that are required for cell migrations and binary cell fate decisions associated with asymmetric cell division, respectively; in yeast two-hybrid assays, the POP-1 N-terminal beta-catenin binding domain interacts with BAR-1/beta-catenin as well as with the more divergent beta-catenin, SYS-1; when coexpressed with SYS-1, POP-1 is able to activate transcription from a promoter with TCF binding sites; during development, maternally provided POP-1 is first detected in the nuclei of maturing oocytes and then in nearly all cells of the early embryo; in sister blastomeres in the early embryo, POP-1 is detected at lower levels in posterior blastomeres, such as E and P3, than in corresponding anterior blastomeres, MS and C; in later developmental stages, POP-1 is detected in a subset of tissues including hypodermal seam cells, gonadal precursors, and the developing vulva; in the vulva, POP-1 also exhibits an asymmetric staining pattern, with sister cells showing high or low levels of POP-1 depending upon their orientation along the anterior/posterior axis of the vulva.
GO Process (22)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
- RNA interference [IMP]
- Wnt signaling pathway [TAS]
- apoptotic process [IMP]
- asymmetric cell division [IMP]
- canonical Wnt signaling pathway [IBA]
- embryo development ending in birth or egg hatching [IMP]
- embryonic pattern specification [IGI, IMP]
- hermaphrodite genitalia development [IMP]
- mesodermal cell fate determination [IGI, IMP]
- mitotic spindle organization [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of vulval development [IMP]
- neuron development [IMP]
- pharyngeal muscle development [IGI, IMP]
- polarity specification of proximal/distal axis [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of vulval development [IMP]
- regulation of asymmetric cell division [IGI]
- regulation of cell fate specification [IGI]
- regulation of defecation rhythm [IMP]
- regulation of transcription from RNA polymerase II promoter [ISS]
- reproduction [IMP]
Gene Ontology Molecular Function- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- beta-catenin binding [IPI]
- histone acetyltransferase binding [IPI]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription factor binding [IPI]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- beta-catenin binding [IPI]
- histone acetyltransferase binding [IPI]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription factor binding [IPI]
Caenorhabditis elegans
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global analysis of genetic interactions in Caenorhabditis elegans.
BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]
J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]
Quantitative Score
- 4.0 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: organism development variant (WBPHENOTYPE:0000531)
Additional Notes
- A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
- Negative Genetic
Curated By
- BioGRID