BAIT
SOS-1
CELE_T28F12.3, let-341, T28F12.3
sos-1 encodes an ortholog of Son of sevenless, a guanine nucleotide exchange factor; sos-1 is required for viability, sex myoblast migration, vulval induction, and oogenesis; sos-1 acts genetically downstream of let-23 and upstream of let-60 with respect to vulval development; SOS-1 binds bovine calmodulin in vitro in a calcium-dependent manner.
GO Process (10)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
- body morphogenesis [IMP]
- cell migration [IMP]
- embryo development [IMP]
- embryo development ending in birth or egg hatching [IMP]
- female gamete generation [IMP]
- oviposition [IMP]
- positive regulation of Ras GTPase activity [ISS]
- positive regulation of vulval development [IGI]
- reproduction [IMP]
- secretion by cell [IMP]
Gene Ontology Molecular Function
Caenorhabditis elegans
PREY
POP-1
CELE_W10C8.2, sys-2, W10C8.2
pop-1 encodes an HMG box-containing protein that is the sole C. elegans member of the TCF/LEF family of transcription factors; in C. elegans, POP-1 functions as a component of the canonical and noncanonical Wnt signaling pathways that are required for cell migrations and binary cell fate decisions associated with asymmetric cell division, respectively; in yeast two-hybrid assays, the POP-1 N-terminal beta-catenin binding domain interacts with BAR-1/beta-catenin as well as with the more divergent beta-catenin, SYS-1; when coexpressed with SYS-1, POP-1 is able to activate transcription from a promoter with TCF binding sites; during development, maternally provided POP-1 is first detected in the nuclei of maturing oocytes and then in nearly all cells of the early embryo; in sister blastomeres in the early embryo, POP-1 is detected at lower levels in posterior blastomeres, such as E and P3, than in corresponding anterior blastomeres, MS and C; in later developmental stages, POP-1 is detected in a subset of tissues including hypodermal seam cells, gonadal precursors, and the developing vulva; in the vulva, POP-1 also exhibits an asymmetric staining pattern, with sister cells showing high or low levels of POP-1 depending upon their orientation along the anterior/posterior axis of the vulva.
GO Process (22)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
- RNA interference [IMP]
- Wnt signaling pathway [TAS]
- apoptotic process [IMP]
- asymmetric cell division [IMP]
- canonical Wnt signaling pathway [IBA]
- embryo development ending in birth or egg hatching [IMP]
- embryonic pattern specification [IGI, IMP]
- hermaphrodite genitalia development [IMP]
- mesodermal cell fate determination [IGI, IMP]
- mitotic spindle organization [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of vulval development [IMP]
- neuron development [IMP]
- pharyngeal muscle development [IGI, IMP]
- polarity specification of proximal/distal axis [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of vulval development [IMP]
- regulation of asymmetric cell division [IGI]
- regulation of cell fate specification [IGI]
- regulation of defecation rhythm [IMP]
- regulation of transcription from RNA polymerase II promoter [ISS]
- reproduction [IMP]
Gene Ontology Molecular Function- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- beta-catenin binding [IPI]
- histone acetyltransferase binding [IPI]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription factor binding [IPI]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- beta-catenin binding [IPI]
- histone acetyltransferase binding [IPI]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription factor binding [IPI]
Caenorhabditis elegans
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global analysis of genetic interactions in Caenorhabditis elegans.
BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]
J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]
Quantitative Score
- 3.4286 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: organism development variant (WBPHENOTYPE:0000531)
Additional Notes
- A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
- Negative Genetic
Curated By
- BioGRID