BAIT
EGL-15
CELE_F58A3.2
Protein EGL-15
GO Process (17)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- axon guidance [IMP]
- cell migration [IMP]
- digestive tract morphogenesis [IMP]
- embryo development ending in birth or egg hatching [IMP]
- engulfment of apoptotic cell [IMP]
- fibroblast growth factor receptor signaling pathway [IMP]
- hermaphrodite genitalia development [IMP]
- locomotion [IMP]
- muscle organ development [IMP]
- nematode larval development [IMP]
- oviposition [IMP]
- protein catabolic process [IMP]
- protein phosphorylation [IDA]
- receptor-mediated endocytosis [IMP]
- regulation of cell projection organization [IMP]
- reproduction [IMP]
- secretion by cell [IMP]
Gene Ontology Molecular Function
Caenorhabditis elegans
PREY
GSK-3
CELE_Y18D10A.5, sgg-1, Y18D10A.5
gsk-3 encodes the C. elegans glycogen synthase kinase ortholog; during embryonic development, GSK-3 functions in the Wnt signaling pathway that restricts specification of mesendodermal tissue to the appropriate blastomere; GSK-3 also functions in a Wnt pathway that regulates anteroposterior axon guidance; GSK-3 plays a role in regulating the oocyte-to-embryo transition, by phosphorylating and negatively regulating the OMA-1 zinc finger protein, and in regulation of the oxidative stress response pathway by phosphorylating SKN-1, thereby excluding it from intestinal nuclei; GSK-3, along with MOM-5/Frizzled and APR-1/APC is also required for distal tip cell migration in the gonad and for the engulfment of apoptotic cells, indicating that the Wnt pathway signals to CED-10/Rac to regulate cytoskeletal rearrangement during different cellular processes; GSK-3 can be phosphorylated by murine ERK2 in vitro, suggesting that it is a substrate for the RTK-RAS-ERK pathway in vivo; consistent with this, GSK-3 phosphorylation is absent in mpk-1 mutant animals.
GO Process (13)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- Wnt signaling pathway, regulating spindle positioning [IMP]
- cell migration [IMP]
- embryo development ending in birth or egg hatching [IMP]
- engulfment of apoptotic cell [IMP]
- gene expression [IMP]
- germ cell development [IGI]
- gonad development [IMP]
- hatching [IMP]
- locomotion [IMP]
- mitotic spindle organization [IMP]
- negative regulation of Wnt signaling pathway [IMP]
- nematode larval development [IMP]
- receptor-mediated endocytosis [IMP]
Gene Ontology Molecular Function
Caenorhabditis elegans
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global analysis of genetic interactions in Caenorhabditis elegans.
BACKGROUND: Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here, we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the ... [more]
J. Biol. Sep. 28, 2007; 6(3);8 [Pubmed: 17897480]
Quantitative Score
- 2.625 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: organism development variant (WBPHENOTYPE:0000531)
Additional Notes
- A systematic genetic interaction analysis (SGI) was carried out to detect interactions between 11 query mutants and 858 target genes compromised by RNA interference (RNAi). Interactions were determined using growth scores that indicated whether the resulting number of progeny from the double mutant was significantly different than that of single mutant controls.
- Negative Genetic
Curated By
- BioGRID