EHMT2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UHRF1
Gene Ontology Biological Process
- cell proliferation [IEP]
- histone monoubiquitination [ISS]
- histone ubiquitination [IDA]
- maintenance of DNA methylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity [IC]
- positive regulation of cellular protein metabolic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IC]
- protein autoubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function- core promoter proximal region sequence-specific DNA binding [IDA]
- hemi-methylated DNA-binding [IDA]
- histone binding [IDA]
- identical protein binding [ISS]
- methyl-CpG binding [IDA]
- methylated histone binding [IDA]
- nucleosomal histone binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- ubiquitin-protein transferase activity [IDA, ISS]
- zinc ion binding [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- hemi-methylated DNA-binding [IDA]
- histone binding [IDA]
- identical protein binding [ISS]
- methyl-CpG binding [IDA]
- methylated histone binding [IDA]
- nucleosomal histone binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- ubiquitin-protein transferase activity [IDA, ISS]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
UHRF1 binds G9a and participates in p21 transcriptional regulation in mammalian cells.
UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) is a multi-domain protein associated with cellular proliferation and epigenetic regulation. The UHRF1 binds to methylated CpG dinucleotides and recruits transcriptional repressors DNA methyltransferase 1 (DNMT1) and histone deacetylase 1 (HDAC1) through its distinct domains. However, the molecular basis of UHRF1-mediated transcriptional regulation via chromatin modifications is yet to be fully ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UHRF1 EHMT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EHMT2 UHRF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EHMT2 UHRF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EHMT2 UHRF1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID