An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Barrier-to-autointegration factor proteome reveals chromatin-regulatory partners.

Montes de Oca R, Shoemaker CJ, Gucek M, Cole RN, Wilson KL

Nuclear lamin filaments and associated proteins form a nucleoskeletal ("lamina") network required for transcription, replication, chromatin organization and epigenetic regulation in metazoans. Lamina defects cause human disease ("laminopathies") and are linked to aging. Barrier-to-autointegration factor (BAF) is a mobile and essential component of the nuclear lamina that binds directly to histones, lamins and LEM-domain proteins, including the inner nuclear membrane ... [more]

PLoS ONE Sep. 18, 2009; 4(9);e7050 [Pubmed: 19759913]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BANF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

TUFM