PRMT5
Gene Ontology Biological Process
- circadian regulation of gene expression [IMP]
- endothelial cell activation [ISO]
- histone H4-R3 methylation [IMP]
- histone arginine methylation [IBA]
- negative regulation of cell differentiation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- peptidyl-arginine N-methylation [ISO]
- peptidyl-arginine methylation [ISO]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IBA, ISO]
- positive regulation of oligodendrocyte differentiation [ISO]
- protein methylation [ISO]
- regulation of DNA methylation [ISO]
- regulation of ERK1 and ERK2 cascade [ISO]
- spliceosomal snRNP assembly [ISO]
Gene Ontology Molecular Function- chromatin binding [IMP]
- core promoter sequence-specific DNA binding [IDA]
- histone-arginine N-methyltransferase activity [IBA]
- methyltransferase activity [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein-arginine omega-N symmetric methyltransferase activity [ISO]
- ribonucleoprotein complex binding [ISO]
- transcription corepressor activity [IMP]
- chromatin binding [IMP]
- core promoter sequence-specific DNA binding [IDA]
- histone-arginine N-methyltransferase activity [IBA]
- methyltransferase activity [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein-arginine omega-N symmetric methyltransferase activity [ISO]
- ribonucleoprotein complex binding [ISO]
- transcription corepressor activity [IMP]
MYOD1
Gene Ontology Biological Process
- cardiac muscle cell differentiation [NAS]
- cellular response to estradiol stimulus [IDA]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to oxygen levels [IDA]
- cellular response to starvation [IDA]
- histone H3 acetylation [IDA]
- histone H4 acetylation [IDA]
- muscle organ development [IDA, IGI]
- myoblast differentiation [IDA]
- myoblast fate determination [IDA]
- myoblast fusion [IMP]
- myotube cell development [ISO]
- myotube differentiation [IDA]
- myotube differentiation involved in skeletal muscle regeneration [IMP]
- negative regulation of myoblast proliferation [IMP]
- positive regulation of muscle cell differentiation [ISO]
- positive regulation of myoblast fusion [ISO]
- positive regulation of skeletal muscle tissue regeneration [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of RNA splicing [ISO]
- regulation of alternative mRNA splicing, via spliceosome [IDA, IMP]
- regulation of transcription from RNA polymerase II promoter [IDA, TAS]
- skeletal muscle cell differentiation [IMP, NAS]
- skeletal muscle fiber adaptation [IMP]
- skeletal muscle fiber development [IMP]
- skeletal muscle tissue development [IDA, IGI, IMP, TAS]
- striated muscle cell differentiation [IMP]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The protein arginine methyltransferase Prmt5 is required for myogenesis because it facilitates ATP-dependent chromatin remodeling.
Skeletal muscle differentiation requires the coordinated activity of transcription factors, histone modifying enzymes, and ATP-dependent chromatin remodeling enzymes. The type II protein arginine methyltransferase Prmt5 symmetrically dimethylates histones H3 and H4 and numerous nonchromatin proteins, and prior work has implicated Prmt5 in transcriptional repression. Here we demonstrate that MyoD-induced muscle differentiation requires Prmt5. One of the first genes activated during ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MYOD1 PRMT5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID