POU5F1
Gene Ontology Biological Process
- BMP signaling pathway involved in heart induction [ISO]
- blastocyst growth [IGI]
- cardiac cell fate determination [ISO]
- cell fate commitment [IMP]
- cell fate commitment involved in formation of primary germ layer [ISO]
- ectodermal cell fate commitment [IDA]
- endodermal cell fate commitment [IDA]
- endodermal cell fate specification [ISO]
- germ-line stem cell maintenance [IMP]
- mRNA transcription from RNA polymerase II promoter [IDA]
- mesodermal cell fate commitment [IDA]
- negative regulation of calcium ion-dependent exocytosis [IDA]
- negative regulation of cell differentiation [IGI]
- negative regulation of gene silencing by miRNA [ISO]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of gene expression [ISO]
- regulation of heart induction by regulation of canonical Wnt signaling pathway [ISO]
- regulation of methylation-dependent chromatin silencing [ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA]
- stem cell maintenance [IMP]
- transcription from RNA polymerase II promoter [IDA, ISO]
- trophectodermal cell differentiation [IGI]
Gene Ontology Molecular Function- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
SALL1
Gene Ontology Biological Process
- branching involved in ureteric bud morphogenesis [IMP]
- embryonic digestive tract development [ISO]
- embryonic digit morphogenesis [IMP, ISO]
- forelimb morphogenesis [IGI]
- heart development [ISO]
- hindlimb morphogenesis [IGI]
- inductive cell-cell signaling [IMP]
- kidney development [ISO]
- kidney epithelium development [IMP]
- limb development [ISO]
- mesenchymal stem cell maintenance involved in metanephric nephron morphogenesis [IMP]
- mesenchymal to epithelial transition involved in metanephros morphogenesis [IMP]
- negative regulation of ectoderm development [IMP]
- negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis [IMP]
- negative regulation of mesoderm development [IMP]
- negative regulation of smoothened signaling pathway [IGI]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- negative regulation of transcription, DNA-templated [IDA, IMP, ISO]
- neural tube closure [IGI]
- neural tube development [IGI]
- olfactory bulb development [IMP]
- olfactory bulb interneuron differentiation [IMP]
- olfactory bulb mitral cell layer development [ISO]
- olfactory nerve development [IMP]
- outer ear morphogenesis [ISO]
- positive regulation of Wnt signaling pathway [ISO]
- positive regulation of branching involved in ureteric bud morphogenesis [IMP]
- positive regulation of neuron differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISO]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of neural precursor cell proliferation [IMP]
- transcription from RNA polymerase II promoter [IDA]
- ureteric bud development [IMP]
- ureteric bud invasion [IMP]
- ventricular septum development [IGI, IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [IDA]
- RNA polymerase II transcription factor binding [IPI]
- beta-catenin binding [ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- histone deacetylase activity [IMP]
- protein binding [IPI]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [IDA]
- RNA polymerase II transcription factor binding [IPI]
- beta-catenin binding [ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- histone deacetylase activity [IMP]
- protein binding [IPI]
Gene Ontology Cellular Component
- NuRD complex [IDA]
- chromocenter [ISO]
- cytoplasm [ISO]
- heterochromatin [IDA, ISO]
- nucleus [IC, IDA, ISO]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
An Oct4-centered protein interaction network in embryonic stem cells.
Transcription factors, such as Oct4, are critical for establishing and maintaining pluripotent cell identity. Whereas the genomic locations of several pluripotency transcription factors have been reported, the spectrum of their interaction partners is underexplored. Here, we use an improved affinity protocol to purify Oct4-interacting proteins from mouse embryonic stem cells (ESCs). Subsequent purification of Oct4 partners Sall4, Tcfcp2l1, Dax1, and ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POU5F1 SALL1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 674413 | |
| POU5F1 SALL1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 471818 | |
| POU5F1 SALL1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID