An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

An Oct4-centered protein interaction network in embryonic stem cells.

van den Berg DL, Snoek T, Mullin NP, Yates A, Bezstarosti K, Demmers J, Chambers I, Poot RA

Transcription factors, such as Oct4, are critical for establishing and maintaining pluripotent cell identity. Whereas the genomic locations of several pluripotency transcription factors have been reported, the spectrum of their interaction partners is underexplored. Here, we use an improved affinity protocol to purify Oct4-interacting proteins from mouse embryonic stem cells (ESCs). Subsequent purification of Oct4 partners Sall4, Tcfcp2l1, Dax1, and ... [more]

Cell Stem Cell Apr. 02, 2010; 6(4);369-81 [Pubmed: 20362541]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ESRRB

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]protein binding [IPI]sequence-specific DNA binding [ISO]transcription coactivator activity [IDA]transcription factor binding [IPI]

Gene Ontology Cellular Component

FKBP15